Cellular Cardiovascular Function Laboratory
Description of Research
Heart disease remains the leading cause of death of aged women in the United States and has been directly linked to age-related estrogen deficiency. Our research focuses on animal models of menopause, ischemia-reperfusion injury, metabolic syndrome, and cardioprotection. Specifically, we study signaling mechanisms involved in conferring cardioprotection via estrogen receptors, protein kinase C (PKCe), metabolic enzymes, adipose tissue, and exercise in aged female rodents. Additionally, the effects of chronic alcohol consumption, aging and estrogen deficiency on cardioprotective pathways are being studied.
From left to right (top row): Donna Korzick, Craig Hunter, Mary Hutchinson, Jennifer Miller-Lee, Rick Ball (bottom row): Stephanie Eldred, Sarah Jefferson, Nanette Tomicek
Director: Donna H. Korzick
Research Associate: J. Craig Hunter
Senior Research Technologist: Mary C. Hutchinson
Senior Research Assistant: Rick W. Ball
Postdoctoral Fellow: Jennifer L. Miller-Lee
Graduate Student: Nanette J. Tomicek
Honors Undergraduate Students: Sarah J. Jefferson, Stephanie V. Eldred
- Tomicek NJ, TS Lancaster, DH Korzick. Dysregulated Estrogen Receptor ß Signaling in Adipose Tissue Increases Obesity and Metabolic Risk in Aged Female Rats, in review, Steroid Biochemistry and Molecular Biology, July 2009.
- Novotny JL, AM Simpson, NJ Tomicek, TL Lancaster, and DH Korzick. Rapid Estrogen Receptor-a Activation Improves Ischemic Tolerance in Aged Female Rats Through a Novel PKCe-Dependent Mechanism. Endocrinology. 2009; 150:889-96 (Epub 2008 Oct 16).
- Korzick DH, JC Kostyak, JC Hunter, and KW Saupe. Local Delivery of PKCe-Activating Peptide Mimics Ischemic Preconditioning in Aged Hearts Through GSK3ß but not F1ATPase Inactivation. Am J Physiol, 2007; 293: H2056-H2063 (Epub 2007 Aug 3).
- Bowles, DK, KK Maddali, DL Tharp, and DH Korzick. PKCd mediates anti-proliferative, pro-apoptotic effects of testosterone on coronary smooth muscle. Am J Physiol, 2007; 293: C805-C813 (Epub 2007 May 16).
- Hunter JC, JC Kostyak, JL Novotny, AM Simpson, and DH Korzick. Estrogen deficiency decreases ischemic tolerance in aged rat myocardium: roles of PKCd, PKCe, Akt, and GSK3ß. Am J Physiol, 2007; 292: R800-R809 (Epub 2006 Sept 28).
- Boluyt MO, GM Cirrincione, AM Loyd, DH Korzick, JL Parker, and MH Laughlin. Effects of gradual coronary artery occlusion and exercise training on gene expression in swine heart. Mol Cell Biochem, 2007; 294: 87-96 (Epub 2006 Aug 26).
- Klabunde ER, DP Brooks, DH Korzick, D Michele. Navigating the interview: how to make it work for you. Physiologist, 2006; 49: 260-266.
- Kostyak JC, JC Hunter and DH Korzick. Acute PKCd inhibition limits ischemia-reperfusion injury in the aged rat heart: role of GSK3ß. Cardiovasc Res, 2006; 70: 325-334.
- Maddali KK, DH Korzick, DL Tharp and DK Bowles. Testosterone increases coronary smooth muscle L-type calcium channel expression in a PKC? mediated manner. J Biol Chem. 2005; 280:43024-4309.
- Korzick DH, JM Muller-Delp, P Dougherty, CL Heaps, DK Bowels and KK Krick. Exaggerated coronary vasoreacitvity to endothelin-1 in aged rats: role of protein kinase C. Cardiovasc Res. 2005; 66: 384-392.
- Hunter JC and DH Korzick. Age- and sex-dependent alterations in PKC-ERK1/2 signaling in rat myocardium. Mech Ageing Dev. 2005; 66: 384-392.
- Hunter JC and DH Korzick. PKC distribution and translocation in rat myocardium: methodological considerations. J Pharmacol Toxicol Methods. 2005; 51: 129-138.
Current Experimental Approaches
- Langendorff Isolated Heart Perfusion – Studying the role of estrogen receptors on recovery from ischemia/reperfusion
- Western Blotting and Realtime RT-PCR – Uncovering cell signalling pathways that are upregulated or activated to promote cardioprotection, or downregulated by age, estrogen deficiency, or alcohol abuse.
- Adipocyte Cell Culture – Understanding cardioprotective signaling originating from fat tissue and adiponectin, estrogen cross-talk
- State-of-the-Art Proteomic MS/MS Techniques – Discovery-based screening to uncover novel mitochondrial mediators of cardioprotection, downstream of estrogen receptors and PKC; and to discover mitochondrial mediators of damage due to estrogen deficiency, age, or alcohol abuse.
- Mitochondrial and Nuclear Mechanisms of Estrogen Receptor-Mediated CardioprotectionNIH (NHLBI) RO1 HL091097
- Mitochondrial and Nuclear Mechanisms of Estrogen Receptor-Mediated Cardioprotection NIH (NHLBI) ARRA Administrative Supplement HL091097-01A2S1
- Novel Mitochondrial Mechanisms of Ethanol-Induced Cardiac Injury in Aged Females NIH (NIAAA) RC2 AA019403